LifeCanvas SmartSPIM

Product Description

Light sheet microscope optimised for intact tissues

With uniform axial resolution across the whole sample, rapid volumetric imaging, a custom-designed imaging chamber, and up to 6 laser lines (405-785 nm), our SmartSPIM light sheet imaging system provides the accuracy, speed, and flexibility you need to generate the highest quality data. Leveraging axial sweeping technology, SmartSPIM provides homogeneous axial point spread functions (PSFz) across the entire field of view.

SmartSPIM’s high speed volumetric image capture allows you to image an entire mouse brain hemisphere or comparably sized sample in <30 minutes while maintaining exceptional quality resolution. Our custom-designed imaging chamber and sample holders can accommodate a variety of tissue sizes and orientations, from organoids to rat brains to an entire juvenile mouse (2.5 x 2.5 cm standard specimen lateral size, 4 x 7.5 cm extended).

SmartSPIM’s acquisition software has real-time tile correction and is simple to operate, making imaging large samples a breeze, and our SmartAnalytics workstation is tailor-made for crafting publication-ready outputs from SmartSPIM images.

Additional information

Applications & Publications

Publications

• Foster et al. “The Mouse Cortico-Basal Ganglia-Thalamic Network.” Nature (October 2021)
• Muñoz-Castañeda et al. “Cellular Anatomy of the Mouse Primary Motor Cortex.” Nature (October 2021).
• Luchsinger et al. “Delineation of an insula-BNST circuit engaged by struggling behavior that regulates avoidance in mice.” Nature Communications 12, 3561 (June 2021).
• Sweeney et al. “The melanocortin-3 receptor is a pharmacological target for the regulation of anorexia.” Science Translational Medicine 13 (April 2021).
• Albanese et al. “Multiscale 3D Phenotyping of Human Cerebral Organoids.” Scientific Reports 10 (December 2020).
• Park et al. “Protection of Tissue Physicochemical Properties Using Polyfunctional Crosslinkers.” Nature Biotechnology 37 (December 2018).