OptoTIRF V2 Laser Illuminator

• Motorised movement of illumination spot and widefield bypass
• Joystick or software control
• Single or multimode fibre

Product Description

Affordable Motorised Total Internal Reflection Fluorescence and Widefield Illumination

The OptoTIRF V2 is a compact and powerful, yet inexpensive, stepper-motor-controlled TIRF illuminator designed to fit onto any research-grade inverted microscope. It allows a single or multimode laser spot to be focussed anywhere in the back-aperture of an objective lens with joystick or software control, and simple storage and recall of preset positions via digital or COM interface. This makes it suitable for acquisition protocols involving TIRF and / or oblique illumination at a range of penetration depths and wavelengths. Although it lacks the fast scanning functionality of the Gataca iLAS (which we are also proud to distribute), the 360 degree stepper-motor-control does allow the user to illuminate from multiple points during an experiment, making it straightforward to tweak the illumination to the sample and minimise fringes or shading gradients. Field uniformity is further enhanced by a dither function that helps to avoid the artefacts associated with point TIRF. Flexibility is enhanced by a motorised bypass port, for widefield illumination using LEDs or a liquid light guide, or, for the addition of a second TIRF module for truly simultaneous dual-colour imaging.

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Additional information


• Single molecule localisation & tracking
• Kinetic studies of single molecule interactions (i.e. ligand binding, protein: protein and protein: DNA interactions)
• Kinetic studies of proteins (i.e. actin filaments & microtubules)
• Super-resolution techniques (i.e. PALM/STORM) ) for subdiffraction localization of single molecules
• Low-cost, open source EasySTORM

Key Benefits

• Compact modular system for easy integration with other modalities onto any inverted microscope base
• Motorised movement of illumination spot for optimised point TIRF / oblique illumination
• Joystick or software control –simple COM commands or digital control will work with any imaging software
• Motorised widefield bypass mode
• Accommodates singlemode or (small) multimode fibre input for use with Cairn or third-party laser sources
• Integrated variable field stop with X_Y alignment
• Simple optical path suitable for adaptation for custom requirements
• Affordable upgrade for existing frames or laser sources
• Modular collimator allows quick and easy optimisation for field flatness or power density

Additional information

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