Light tight enclosure for sensitive optical recordings. The ALLIGATOR comprises of a high-sensitivity EMCCD camera mounted with high resolution and anti-mist optics enclosed within a custom humidified CO2/N2 170 L tissue culture incubator and light-tight shroud.
The ALLIGATOR can image up to six 96-well plates simultaneously and supports recording from a wide variety of cell types, tissues, and organotypic slices. In addition, the ALLIGATOR affords programmable control of temperature, % CO2, and % O2 allowing long-term bioluminescence recordings under physiologically relevant conditions. The system is fully supported in all commercial software imaging packages (Micromanager, Visiview, NIS-Elements, etc).
“This fantastic new system offers the capacity for high-throughput collection over time scales that vary from seconds to weeks, without sacrificing the quality of the data collected. The flexible and sensitive environmental control gives us the ability to do experiments which would simply not be possible otherwise.”
Dr. John S. O’Neill, MRC Laboratory of Molecular Biology, University of Cambridge
A jove video showing how the ALLIGATOR can be used in the context of circadian rhythms research (thanks to John O’Neill’s lab, Cambridge)
The Alligator system includes the following modifications:
Distinct circadian mechanisms govern cardiac rhythms and susceptibility to arrhythmia – April 2021
Insulin/IGF-1 Drives PERIOD Synthesis to Entrain Circadian Rhythms with Feeding Time – February 2019
Circadian actin dynamics drive rhythmic fibroblast mobilization during wound healing – November 2017
The Pentose Phosphate Pathway Regulates the Circadian Clock – August 2016
In-depth Characterization of Firefly Luciferase as a Reporter of Circadian Gene Expression in Mammalian Cells – October 2016
Cairns’s design ethos is based on compatibility across a wide range of manufacturers. In the rare case where we do not have a compatible part, we have a custom design team available to provide a solution.
We provide a comprehensive 12-month warranty on all our products.
We aim for a delivery time of 2-4 weeks. However, the specific delivery time will be confirmed at the point of order.
We offer full training and support across our entire product range.
Yes, we have full custom design capabilities and a multidisciplinary team of scientists and engineers available to provide a solution that meets your needs.
Our CellCams are supported in Micro-Manager and we have a full SDK available.
