Product Description
Link to RedShirtImaging brochure
Non-destructive read (NDR) mode
DaVinci CMOS imagers can be read non-destructively without resetting the pixels. Thus the pixels can be read repeatedly to reduce noise, or the frame rate can be doubled with any image frame serving as the reset frame for a subsequent image frame. This allows post-acquisition framing of fast events. By contrast, CDS imaging resets the pixels every frame and can cause events to be randomly split between frames as shown below.
NDR for STORM
For STORM imaging, DaVinci CMOS imagers are run in a windowed mode at high frame rates. 2048×180 pixels at 2500 Hz is typical, acquiring 10,000 frames in 4 seconds. The imager is reset once every 500 frames. Sample drift and bleaching are greatly reduced.
Flashes are oversampled and both reset and image frames are optimally selected POST-ACQUISITION to maximize SNR and minimize background.
With slower EMCCD cameras, flashes can begin and end at any time within one or two adjacent frames adding to the uncertainty of when the flash occurred. The signal can be randomly split between frames, reducing the SNR.
