L-SPI Large Selective Plane Illuminator

Product Description

Now complete with X, Y, and Z stages to greatly assist usability 

The L-SPI is designed to work with any macro- or microscope, single-mode fibre laser source and scientific camera. This simple and effective instrument recombines two uniform, wide lightsheets at right angles, reducing shadowing without the need for sample rotation or image fusion. The system includes optional sample chambers, fast long travel piezo stage and a second L-SPI head to bring sheets in from four directions. The L-SPI can be adapted to a wide range of experimental purposes. Its compact size and large freedom of access (e.g. for positioning micro-electrodes) make it a highly flexible solution.

Please visit our technical support pages for a write up by our co-designer Philippe Laissue

Additional information

Applications & Publications

2022

Dyer, L., Parker, A., Paphiti, K., & Sanderson, J. (2022). Lightsheet
microscopy. Current Protocols, 2, e448. doi: 10.1002/cpz1.448

• Assessing phototoxicity in live fluorescence imaging
• LITE microscopy: A technology for high numerical aperture, low photobleaching fluorescence imaging
• Light sheet microscope tackles live coral imaging

Publications

• McNamara 20171227W draft – smFISH and qIF plus optical clearing Li Germain Gerner 2017 PNAS