OptoLED Light Source

Product Description

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The ultra-high stability and “instantaneous” (sub-microsecond) vibration-free switching and intensity modulation are valuable in a wide range of applications such as combined imaging and electrophysiology, voltage sensitive dye studies and high-speed tracking. The facility to “gate” the light source directly from the camera and thus only expose the specimen whilst the camera is integrating helps to reduce photo-bleaching / photo-damage and also prevents bleedthrough during frame readout or rolling shutter artefacts. Ultraviolet and white LEDs are also available for uncaging applications and brightfield illumination. The rapid modulation and ability to accurately define independent illumination areas for different wavelengths makes the OptoLED the ideal source for Optogenetics. The power supply allows devices to be transiently overdriven to 5A to give increased optical intensity whilst fully protecting the LED from damage.

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Additional information

Applications	• Multi-wavelength fluorescence microscopy • Visible/IR transmitted light microscopy • Optogenetics using fibres or epi-illumination • Macro fluorescence imaging/Optical Mapping • Flash photolysis • High speed Fura-2 calcium imaging • New FuraLED coupling available
Key Benefits	• High intensity • Instantaneous vibration-free switching • Long life (should never need replacing) • Variable intensity – no need for ND filters • Minimal unwanted infrared (heat) output • Fully modular system accommodates an ever-expanding range of emitters
Full Specification	• Typical optical power at specimen available on request • Fluorescence LED heads available at 340nm, 365nm, 385nm, 405nm, 440nm**, 455nm, 470nm, 490nm, 505nm, 525nm, 550nm**, 565nm, 590nm, 617nm, 627nm and white with • Single,dual, triple and quad couplings available for Nikon, Olympus, Leica and Zeiss microscopes • TTL-controlled digital switching and analogue voltage-controlled intensity modulation accessed via BNC connections • Maximum output current 1A, 2A or 5A, selectable by internal jumper links • Optical switching times <100nsec • Response time to change external analogue input <10μsec • External analogue control voltage range 0 to +5V / 1V=1A **filtered high power white LED
Testimonials	Our need was to stimulate an isolated photoreceptor cell with sufficient light intensity to isomerize nearly all the photopigment molecules, and do so with the shortest flash duration possible: this would synchronize all such events, summate their electrical effects to maximize detectabililty, and avoid overlap with the photocurrent that is elicited only a few milliseconds later. Even the fastest electromechanical shutters proved inadequate for this task, while discharge flash lamps produced unacceptable electrical interference. We were pleased that the OptoFlash (OptoLED) could pump enough photons within a sub-millisecond flash to even saturate rhodopsin, maintaining an excellent intensity control and defined time course. Enrico Nasi – Cellular dynamics, Woods Hole, MBL