OptoSplit III

The OptoSplit III (TripleSplit) extends the OptoSplit image splitter concept, adding an optional second beamsplitter to split the field into either two or three separate, spatially equivalent, channels, which can be displayed side by side on a single camera chip.

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Splitting is usually performed on the basis of wavelength or polarisation, allowing applications where there is a requirement for simultaneous, or high speed, acquisition of multiple emission bands or polarisations states. The simultaneous acquisition of up to three images offers a major benefit over manual or electronic filter changers, as there is no longer a need to pause acquisition while the filter position is changed. This allows your camera to be operated in fast stream modes.

Device drivers are included in most commercial imaging packages to assist registration and to allow realtime and off-line ratioing or fluorescence overlays. Alternatively the TripleSplit can be used with simple image capture software and the processing carried out manually offline or using our own MicroManager and ImageJ drivers. The simple and accessible design makes the Optosplit III an excellent platform for alternative applications, such as pFRET polarisation, or multi-depth imaging.

Whilst optimised for coupling to a scientific microscope, the Optosplit III image splitters can also be used with camera lenses or any other system of lenses which produce an image plane of suitable size and f/number (please ask for details).

Application Usage

  • Förster Resonance Energy Transfer (FRET)
  • Ratiometric calcium, voltage & pH imaging
  • Simultaneous multi fluorescent probe imaging
  • Polarisation studies (anisotropy)
  • Simultaneous phase contrast / DIC and fluorescence
  • Simultaneous multi Z depth imaging
  • Total Internal Reflection Fluorescence (TIRF)
  • Spinning disk confocal
  • Single Plane Illumination Microscopy (SPIM)
  • 3D super resolution PALM/STORM (using cylindrical lenses)

Features and Benefits

  • Compact design with integral C-mount input and output ports as standard (F and T mount on request)
  • Support for sensors up to 29.4mm diagonal (25mm using single channel cube)
  • Budget friendly alternative to three cameras
  • 425nm to 875nm AR coatings on all optical surfaces
  • 40mm diameter proprietary optics
  • Simple & precise controls for image registration
  • Interchangeable filter / dichroic holders
  • Emission filter dimensions – 25mm diameter
  • Recommended dichroic dimensions – 26x38x2mm (lambda/2 flatness)
  • Single channel ‘Bypass’ cube to easily switch to single channel operation
  • Angled and flat auxiliary drop-in holders for 25mm ND filters and chromatic correction lenses (1 inch on request)
  • 1x and 1.3x magnification available
  • Optional polarisation splitting kit including beamsplitter cube and rotatable polariser
  • Fixed or variable centre fully adjustable rectangular mask to delimit region of interest

Research Examples

Coming Soon!

FAQ

Cairns’s design ethos is based on compatibility across a wide range of manufacturers. In the rare case where we do not have a compatible part, we have a custom design team available to provide a solution. 

We provide a comprehensive 12-month warranty on all our products.

We aim for a delivery time of 2-4 weeks. However, the specific delivery time will be confirmed at the point of order.

We offer full training and support across our entire product range. 

Yes, we have full custom design capabilities and a multidisciplinary team of scientists and engineers available to provide a solution that meets your needs. 

Our CellCams are supported in Micro-Manager and we have a full SDK available. 

Customer Reviews

"The build quality of their equipment is excellent, and has proven both reliable and versatile in our lab’s day-to-day research; our Cairn equipment has provided years of dependable service."
Andrew James
“Over the past 20 years we have purchased a wide range of equipment from Cairn Research, ranging from several Optopatch amplifiers, OptoLED, Optosourse arc lamp and custom-made optical pathways for nanoscale measurements.”
Walter Marcotti
“What I love about this company is the quality and the reliability of every piece of equipment that I purchased. The instruments are made of high-grade materials (mostly aluminum for the optics components) and are intended for heavy everyday use in the laboratory. “
Andreas Werdich

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