• Fast imaging acquisition
• Large field of view
• Compact

Product Description

High-speed Spinning Disk Confocal imaging with up to 22mm FOV

A flexible, compact but full-fledged spinning-disk confocal solution, CICERO makes high-end fluorescence imaging accessible to every laboratory enabling a variety of applications.

CICERO spinning disk solution delivers fast image acquisition speed (15K rpm) and sensitivity, easily enabling live cell imaging and large-scale 3D object imaging. Due to its large field of view (up to 22mm FOV), CICERO offers a minimal scanning process and can capture large samples in a single frame.

CICERO is a reliable and flexible solution compatible with both LED and Laser as illumination sources. Moreover, the wide spectral range enables a large variety of applications. For this, CICERO allows to address both entry-level and challenging applications.


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Additional information

Technical Specifications

Spectral range (confocal/widefield): 400-750nm

Fully automated experiments

Software controlled bypass mode: widefield to confocal

The fastest spinning disk in the market: 15.000 rpm rotation speed, up to kHz range

Choose your disk: 50um pinholes in two different versions based on different pinole spacing

Choose your disk: unique continuous spiral disk makes it faster for high-speed applications

User inspired: customize pinhole size and geometry on request

Exchangeable, synchronized and sealed: the disk box is one of the most versatile features of the new V3. Dust protection and synchronization signals out to the camera make it reliable and faster.

Lateral Resolution (FWHM): ~230 nm (High NA 1.4) diffraction limited

Axial Resolution (FWHM): ~600 nm (High NA 1.4)

High Confocal Quality: this advanced new series brings in a redesigned optical layout optimized to enhance sensitivity and signal-to-noise ratio in any dim and bright conditions

Software controlled filter cubes: 3 positions for simultaneous dual-color imaging

Illumination uniformity: microlenses based for high uniformity across the full field of view

Illumination FOV: different options to match different camera sizes and enhance efficiency

Illumination shaping: preserve your sample. Motorized aperture to cut illumination in the region surrounding the region of interest

Software controlled filter wheels: 4-positions automated cleanup filter wheel, 3-positions automated dichroic filter wheel, 8-positions automated emission filter wheel

Detector Field of View

Ultra-large field of view imaging: maximum 25 mm

Dual-camera view: simultaneous dual-camera view up to 25 mm each camera

illumination FOV: different options to match different camera sizes and enhance efficiency

Pattern Configuration

•CrestOptics Proprietary Disk Pattern Design for high confocal resolution, improved Out of Focus rejection and higher S/N
confocal resolution with 60x NA 1.4 oil immersion Objective: <800nm

•40 micron pinholes

•70 micron pinholes

•Custom pinholes diameters are available on request

•30mm diameter Custom Relay lenses. Diffraction-limited design across all the field of view from high magnification-high NA objectives to low magnification-low numerical aperture obectives

•Fast spinning disk 15.000 RPM disk rotation speed

•Excitation Gimbal mount for easy alignment on custom microscope setup and for best S/N

•Motorized Dichroic Five positions Filter Wheel

•Standard Eight positions Motorized Emission Wheel

•Plug-in spinning disk
•Extraction tools for easy insertion and removal of both dichroic filter and emission filter

•Detector focal plane easy focusing without moving the detector

•Adapter for Multimode Laser or LED SMA-905 fiber excitation